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cellometer vision cba image cytometry system  (Revvity)
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Revvity cellometer vision cba image cytometry system
The effects of DDQ treatment on the viability of HT22 cells. (A) Flow <t>cytometry</t> analysis of cells in four groups; a) Control (HT22 cells), b) Control + DDQ, c) mTau (HT22 cells transfected with mutant Tau cDNA), d) mTau + DDQ. (B) Quantification of cell viability. Increased cell viability levels in DDQ treatment cells when compared with control HT22 cells. However, cell viability was increased in mutant Tau + HT22 cells compared with mTau HT22 cells. Data are expressed as the mean ± SEM of four independent experiments. * P < 0.05, **** P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DDQ: Diethyl (3,4-dihydroxyphenethylamino) (quinolin-4-yl) methylphosphonate; mTau: mutant Tau.
Cellometer Vision Cba Image Cytometry System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effects of DDQ treatment on the viability of HT22 cells. (A) Flow cytometry analysis of cells in four groups; a) Control (HT22 cells), b) Control + DDQ, c) mTau (HT22 cells transfected with mutant Tau cDNA), d) mTau + DDQ. (B) Quantification of cell viability. Increased cell viability levels in DDQ treatment cells when compared with control HT22 cells. However, cell viability was increased in mutant Tau + HT22 cells compared with mTau HT22 cells. Data are expressed as the mean ± SEM of four independent experiments. * P < 0.05, **** P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DDQ: Diethyl (3,4-dihydroxyphenethylamino) (quinolin-4-yl) methylphosphonate; mTau: mutant Tau.

Journal: Neural Regeneration Research

Article Title: Small molecule inhibitor DDQ-treated hippocampal neuronal cells show improved neurite outgrowth and synaptic branching

doi: 10.4103/NRR.NRR-D-24-00157

Figure Lengend Snippet: The effects of DDQ treatment on the viability of HT22 cells. (A) Flow cytometry analysis of cells in four groups; a) Control (HT22 cells), b) Control + DDQ, c) mTau (HT22 cells transfected with mutant Tau cDNA), d) mTau + DDQ. (B) Quantification of cell viability. Increased cell viability levels in DDQ treatment cells when compared with control HT22 cells. However, cell viability was increased in mutant Tau + HT22 cells compared with mTau HT22 cells. Data are expressed as the mean ± SEM of four independent experiments. * P < 0.05, **** P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). DDQ: Diethyl (3,4-dihydroxyphenethylamino) (quinolin-4-yl) methylphosphonate; mTau: mutant Tau.

Article Snippet: HT22 cell endurance using the Cellometer Vision CBA Image Cytometry System (Nexcelom Bioscience LLC, Lawrence, MA, USA), an assay was run to verify the cell viability in all four groups of cells.

Techniques: Flow Cytometry, Control, Transfection, Mutagenesis